Barley ~-amylase/subtilisin Inhibitor. Ii. N-terminal Amino Acid Sequence and Homology with Inhibitors of the Soybean Trypsin Inhibitor (kunitz) Family
نویسنده
چکیده
An inhibitor of barley malt :t-amylase 2 isolated from barley malt and a specific inhibitor of subtilisin isolated from barley have been sequenced by automatic Edman degradation. The sequence of the first 40 N-terminal amino acids is the same for both proteins. The sequence is: Ala-Asp-Pro-Pro-Pro-Val-His-Asp-Thr-Asp-Gly-HisGlu-Leu-Arg-Ala-Asp-Ala-Asn-Tyr-Tyr-Val-Leu-Ser-Ala-Asn-Arg-Ala-His-Gly-GlyX -Leu-Thr-Met-Ala-ProGlyX -Gly-. The ~t-amylase/subtilisin inhibitor is homologous with Kunitz-type protease inhibitors of leguminous seeds. It is also homologous with a rice subtilisin inhibitor (16 of the 24 residues sequenced in identical positions).
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